000 020520000a22003130004500
001 002353
005 20230526120534.0
046 _j19710000
100 _aWilley, Ruth L
245 _aMicrotechniques: A laboratory guide
260 _bThe Macmillan Company
260 _c1971
270 _bNew York
270 _dUS
300 _a99 p.
300 _btabls., diagrs., ilus.
505 _aTissue-processing directions.
505 _aGeneral routine for preparation of tissues: Fixation; fixatives; dissection and fixation of mouse tissues; general paraffin routine; specific paraffin routine for plant tissues; celloidin embedding routine; peterfi's celloidin preimpregnation techniques; gelatine embedding for frozen sections.
505 _aMicrotomes: Rotary microtome for paraffin sections; sliding microtome for celloidin sections; microtomes for frozen sections; the microtome knife.
505 _aStaining: Stains; general staining routine; methylene blue - phloxine; delafield's hematoxylin and eosin Y for paraffin, celloidin, and frozen sections; heidenhain's iron haematoxylin; safranin and fast green; periodic acid-schiff method for glycogen; feulgen method for DNA; altmann's aniline-acid fuchsin for mitochondria; holmes' silver nitrate method for nerves; heidenhain's azan; sudan black B for lipids.
505 _aWhole mounts: General remarks; wright's stain for blood smears; maceration techniques for hydra; maceration technique for hydra; maceration of onion root tips; whole mounts of frog parasites; maceration of wood fibers.
505 _ahe microscope: Optics; optics of the light microscope; adjusting the light microscope; function of the objective and ocular; instructions for a drawing.
505 _aSlide analysis: Cell identification in different tissues; ultrastructure of the cell; slide analysis questions.
505 _aAppendixes: General remarks on primary fixatives; miscellaneous formulae.
546 _aInglés
650 _aTÉCNICAS HISTOLOGÍCAS
852 _aQH207
_bW5
942 _cLB
999 _c4841
_d4841