000 | 020520000a22003130004500 | ||
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001 | 002353 | ||
005 | 20230526120534.0 | ||
046 | _j19710000 | ||
100 | _aWilley, Ruth L | ||
245 | _aMicrotechniques: A laboratory guide | ||
260 | _bThe Macmillan Company | ||
260 | _c1971 | ||
270 | _bNew York | ||
270 | _dUS | ||
300 | _a99 p. | ||
300 | _btabls., diagrs., ilus. | ||
505 | _aTissue-processing directions. | ||
505 | _aGeneral routine for preparation of tissues: Fixation; fixatives; dissection and fixation of mouse tissues; general paraffin routine; specific paraffin routine for plant tissues; celloidin embedding routine; peterfi's celloidin preimpregnation techniques; gelatine embedding for frozen sections. | ||
505 | _aMicrotomes: Rotary microtome for paraffin sections; sliding microtome for celloidin sections; microtomes for frozen sections; the microtome knife. | ||
505 | _aStaining: Stains; general staining routine; methylene blue - phloxine; delafield's hematoxylin and eosin Y for paraffin, celloidin, and frozen sections; heidenhain's iron haematoxylin; safranin and fast green; periodic acid-schiff method for glycogen; feulgen method for DNA; altmann's aniline-acid fuchsin for mitochondria; holmes' silver nitrate method for nerves; heidenhain's azan; sudan black B for lipids. | ||
505 | _aWhole mounts: General remarks; wright's stain for blood smears; maceration techniques for hydra; maceration technique for hydra; maceration of onion root tips; whole mounts of frog parasites; maceration of wood fibers. | ||
505 | _ahe microscope: Optics; optics of the light microscope; adjusting the light microscope; function of the objective and ocular; instructions for a drawing. | ||
505 | _aSlide analysis: Cell identification in different tissues; ultrastructure of the cell; slide analysis questions. | ||
505 | _aAppendixes: General remarks on primary fixatives; miscellaneous formulae. | ||
546 | _aInglés | ||
650 | _aTÉCNICAS HISTOLOGÍCAS | ||
852 |
_aQH207 _bW5 |
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942 | _cLB | ||
999 |
_c4841 _d4841 |