Quantitatively standardize complement-fixation methods for critical evaluation of antigens prepared from Trypanosoma cruzi

Por: Colaborador(es): Series Scientific Publication. 319 Detalles de publicación: Pan American Health Organization. Pan American Sanitary BureauWorld Health Organization. Regional Office for Europe Copenhagen; 1976Descripción: 86 p; tabls., grafsTema(s):
Contenidos:
Standardization of reagents: Sheep cell suspension; antisheep cell hemolysin; complement
Processing and handling of human serum
Preservation and storage of antigens
Determination of the quantitative relationships between serum, antigen, and complement; Selection of the maximally reactive dose of serum or antigen from inspection of isofixation curves
Evaluation of antigens: General; nonspecific reactivity: Qualitative tests with non-chagasic sera; specific reactivity
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Standardization of reagents: Sheep cell suspension; antisheep cell hemolysin; complement

Processing and handling of human serum

Preservation and storage of antigens

Determination of the quantitative relationships between serum, antigen, and complement; Selection of the maximally reactive dose of serum or antigen from inspection of isofixation curves

Evaluation of antigens: General; nonspecific reactivity: Qualitative tests with non-chagasic sera; specific reactivity

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