Microtechniques: A laboratory guide

Tissue-processing directions.

General routine for preparation of tissues: Fixation; fixatives; dissection and fixation of mouse tissues; general paraffin routine; specific paraffin routine for plant tissues; celloidin embedding routine; peterfi's celloidin preimpregnation techniques; gelatine embedding for frozen sections.

Microtomes: Rotary microtome for paraffin sections; sliding microtome for celloidin sections; microtomes for frozen sections; the microtome knife.

Staining: Stains; general staining routine; methylene blue - phloxine; delafield's hematoxylin and eosin Y for paraffin, celloidin, and frozen sections; heidenhain's iron haematoxylin; safranin and fast green; periodic acid-schiff method for glycogen; feulgen method for DNA; altmann's aniline-acid fuchsin for mitochondria; holmes' silver nitrate method for nerves; heidenhain's azan; sudan black B for lipids.

Whole mounts: General remarks; wright's stain for blood smears; maceration techniques for hydra; maceration technique for hydra; maceration of onion root tips; whole mounts of frog parasites; maceration of wood fibers.

he microscope: Optics; optics of the light microscope; adjusting the light microscope; function of the objective and ocular; instructions for a drawing.

Slide analysis: Cell identification in different tissues; ultrastructure of the cell; slide analysis questions.

Appendixes: General remarks on primary fixatives; miscellaneous formulae.

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